For Cosmic Fish, the first arc is only going to focus on about 10 characters more or less, although there's 16-17 in total.
But the story has a total of about 32 if I remember right.
100 is impressive though! I have a comic that has about 60 planned but I've cut it down or mixed characters into one to try and avoid quantity > quality like Bleach or Homestuck ended up having. Not that it's always the case, it's just playing character creation hard mode imo, and rather focus on the story. If done well, then great job!