Comic writer here (sorta, not really..)
My comic would have family and trust as the main themes, but they kinda came up unintentionally? I didn't write it from the get-go with the idea of basing the plot off of them. It's only when I stepped back after a while that I was like "Huh, I guess if you had to put it a certain way.. those ideas would sum it up pretty well."
I wanted to base it off of teamwork, but the story kinda gravitated towards one character's development. It's a duo, but it's basically the story of one of them being supported by the other to the point of changing her. Which I guess goes back to teamwork, but as an unofficial secondary theme lol