Well, on one page (I can't find which now! >.<), my readers started "singing" an adapted version of Bohemian Rhapsody, changing words to fit my comic, in a collaborative little comment-thread that makes me giggle every time someone adds to it.
Also, the first time I introduced Wen, three readers had the following conversation:
1 Whoa, those are some nice thighs o///o
2 she could crush my head with her legs and I would die happy
3 That's a very strange and specific fetish, but I don't disapprove.
and while sex-related comments often make me uncomfortable (hi, I'm on the ace-spectrum), this one just made me laugh. XD