To tell you the truth: I rarely think about what my target audience are when I create my story.
I make the story the way I want, however I do try to make my story more simple & universal to draw in readers. The story has teenage protagonist and it's a lot like shounen manga, but tells in a art-house movie kind of way. It's not pulpy that's for sure and I hope my readers realize that.
My comic does not contain clear romance so it doesn't limit out readers who only like straight romance or BL etc. Also doesn't have very strong sense of male/female gaze (except some comical characters) Also I don't want to focus on character representations, just be story oriented. (aha this may limited out some readers too)
I do try to be as approachable to most readers as possible but in the context that they have to be able to stomach my anthro designs & the story (and also my not so good English, which could be a welcoming thing for international readers
) Also when future chapters where things go dark, I may lose some readers too. that's understandable and I'll be okay with it.
Most of all one just have to show off the uniqueness of your work (within rules), and do so confidently. There will be audience who like your stuff.